GSM5518542: HCT116 ChIPseq SMC3 rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SMC3
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
HCT116
cell type
colon cancer cell line
treatment
none
Sex
male
antibody
anti-SMC3 (abcam:ab9263)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
All ChIP-seq experiments used the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 from Cell Signaling Technologies following the manufacturer's recommended protocol. Four million cells per IP. MNase digested chromatin was pooled into a single tube for brief sonication to lyse nuclei. Supernatant was then split evenly between IPs (minus 2% input). Antibodies and chromatin were incubated overnight at 4°C, rotating. DNA was purified using spin columns. Libraries were prepared using NEB UltraII DNA library kit